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Zan Mitrev's Team

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Sanja Mehandziska

Molecular biologist

Place of birth:

Berovo, Macedonia


02.2017 – to date Molecular Biologist in the Laboratory of Genetics and Personalized Medicine,

Zan Mitrev Clinic, Skopje

02.2012 – to date Ph.D. in Biochemistry,

Specialization area: Molecular Genetics, in the laboratory of Prof. Dr. Georgi Muskhelishvili

Jacobs University Bremen, Bremen, Germany

09.2016 – 02.2017 Intern in the Laboratory of Genetics and Personalized Medicine

Special Hospital for Surgical Diseases Filip Vtori, Skopje

04.2016 – 09.2016 Intern in the Biochemical Laboratory

Special Hospital for Surgical Diseases Filip Vtori, Skopje

03.2011 – 01.2015 Teaching Assistant in the Advanced Genetics Lab Course

Jacobs University Bremen, Bremen, Germany

06.2008 – 08.2008 Intern in the Molecular Genetics department, in the laboratory of Dr. Daniel Peeper

The Netherlands Cancer Institute (NKI-AVL), Amsterdam, Netherlands

07.2007 – 05.2009 Student laboratory assistant

Jacobs University Bremen, Bremen, Germany

11.2012 – 04.2015 Resident Associate

College Nordmetall, Jacobs University Bremen, Bremen, Germany

09.2009 – 01.2012 Master of Science in Molecular Life Sciences

First specialization area: Molecular Genetics, Second specialization area: Cellular and Molecular Biology

Jacobs University Bremen, Bremen, Germany

09.2006 – 06.2009 Bachelor of Science in Biochemistry and Cell Biology

Jacobs University Bremen, Bremen, Germany

Diagnostics ▪  Isolation of nucleic acids from blood, tissue and other biological samples, ct DNA isolation from liquid biopsy

▪  Genotyping and pharmacogenetics analyses with real time polymerase chain reaction (PCR)

▪  Genotyping pharmacogenetics analyses with StripAssays

▪  Typization and quantification of infective microorganisms by real time PCR

▪  Bioinformatic analyses and interpretation of whole exome, clinical exome and gene panels

▪  Genetic counseling

▪  Biochemical analyses

Research ▪  Molecular cloning

▪  Bacterial phage transduction

▪  PCR and qRT-PCR

▪  In vitro transcription

▪  Gel shift assays, EMSA (Electrophoretic Mobility Shift Assay)

▪  Bacterial growth in flasks, in microplate reader, in fermentor

▪  Protein overexpression, extraction and purification by affinity and ion-exchange chromatography using HPLC (High Pressure Liquid Chromatography)

▪  Immunoblotting (Western Blot)

▪  Co-immunoprecipitation of protein complexes

▪  2D Blue Native-PAGE – SDS-PAGE

▪  2D IsoElectric Focusing  (IEF) – SDS-PAGE

▪  Enzymatic activity assays

▪  MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization – Time Of Flight) mass spectrometry

▪  Antibiotic resistance E-test

▪  Swarming assays

▪  Methylation assays

▪  Mouse killing by perfusion, dissection, tissue homogenization

▪  Cryosectioning

▪  Microscopy

▪  Confocal Laser Microscopy

▪  Mammalian cell culture: generation of retroviruses, transduction, colony formation assays, long-term growth curves, microscopy, FACS analysis (Fluorescence-Activated Cell Sorting), BrdU assay

Conferences and seminars

06.2018 European Human Genetics Conference in conjunction with the European Meeting on Psychosocial Aspects of Genetics, 16-19 June, Milano, Italy
03.2018 Workshop on “Next Generation Diagnostics”, 22-24 March, Skopje, Macedonia
01.2018 COMBACTE Good Clinical Practice (GCP) Course, 1-31 January, CLIN-Net (online course)
11.2017 VI Conference for Rare Diseases in South-eastern Europe, 11 November 2017, Skopje, Macedonia (e-poster presentation)
10.2017 NUTRICON Congress 2017, 5-7 October, 2017, Skopje, Macedonia
09.2017 CoMBoS – 1st Congress of Molecular Biologists of Serbia, 20-22 September 2017, Belgrade, Serbia
06.2014 The Biology and Physics of Bacterial Chromosomes, 16-18 June 2014, Birmingham, UK (poster presentation)
09.2013 qPCR and digital PCR congress, 09-10 September 2013 Lyon, France
03.2013 ESF-EMBO Symposium: Bacterial Networks (bacnet13), 16-21 March 2013, Pultusk, Poland  (poster presentation)
03.2013 MoLife Retreat, 25-26 March 2013, Bad Bevensen, Germany  (oral presentation)
05.2011 MoLife Retreat (Molecular Life Sciences Retreat), 19-21 May 2011, Seefeld, Germany


2018 Stajkovska, A., Mehandziska, S., Stavrevska, M., Jakovleva, K., Nikchevska, N., Mitrev, Z., Kungulovski, I., Zafiroski, Gj, Tasic, V., and Kungulovski, G. (2018) Trio Clinical Exome Sequencing in a Patient With Multicentric Carpotarsal Osteolysis Syndrome: First Case Report in the Balkans. Front. Genet. 9:113.
2017 Mehandziska, S., Petrescu, A.M., and Muskhelishvili, G. (2017) Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins. In: The Bacterial Nucleoid: Methods and Protocols. (ed. Olivier Espeli) MiMB Springer Series
2011 Geertz, M., Travers, A., Mehandziska, S., Sobetzko, P., Janga, S. C., Shimamoto, N. and Muskhelishvili, G. (2011) Structural Coupling between RNA Polymerase Composition and DNA Supercoiling in Coordinating Transcription: a Global Role for the Omega Subunit? MBio, vol. 2 no. 4 e00034-11