Zan Mitrev's Team
Back to DoctorsSanja Mehandziska
Molecular biologist
02.2017 – to date | Molecular Biologist in the Laboratory of Genetics and Personalized Medicine,
Zan Mitrev Clinic, Skopje |
02.2012 – to date | Ph.D. in Biochemistry,
Specialization area: Molecular Genetics, in the laboratory of Prof. Dr. Georgi Muskhelishvili Jacobs University Bremen, Bremen, Germany |
09.2016 – 02.2017 | Intern in the Laboratory of Genetics and Personalized Medicine
Special Hospital for Surgical Diseases Filip Vtori, Skopje |
04.2016 – 09.2016 | Intern in the Biochemical Laboratory
Special Hospital for Surgical Diseases Filip Vtori, Skopje |
03.2011 – 01.2015 | Teaching Assistant in the Advanced Genetics Lab Course
Jacobs University Bremen, Bremen, Germany |
06.2008 – 08.2008 | Intern in the Molecular Genetics department, in the laboratory of Dr. Daniel Peeper
The Netherlands Cancer Institute (NKI-AVL), Amsterdam, Netherlands |
07.2007 – 05.2009 | Student laboratory assistant
Jacobs University Bremen, Bremen, Germany |
11.2012 – 04.2015 | Resident Associate
College Nordmetall, Jacobs University Bremen, Bremen, Germany |
09.2009 – 01.2012 | Master of Science in Molecular Life Sciences
First specialization area: Molecular Genetics, Second specialization area: Cellular and Molecular Biology Jacobs University Bremen, Bremen, Germany |
09.2006 – 06.2009 | Bachelor of Science in Biochemistry and Cell Biology
Jacobs University Bremen, Bremen, Germany |
Diagnostics | ▪ Isolation of nucleic acids from blood, tissue and other biological samples, ct DNA isolation from liquid biopsy
▪ Genotyping and pharmacogenetics analyses with real time polymerase chain reaction (PCR) ▪ Genotyping pharmacogenetics analyses with StripAssays ▪ Typization and quantification of infective microorganisms by real time PCR ▪ Bioinformatic analyses and interpretation of whole exome, clinical exome and gene panels ▪ Genetic counseling ▪ Biochemical analyses |
Research | ▪ Molecular cloning
▪ Bacterial phage transduction ▪ PCR and qRT-PCR ▪ In vitro transcription ▪ Gel shift assays, EMSA (Electrophoretic Mobility Shift Assay) ▪ Bacterial growth in flasks, in microplate reader, in fermentor ▪ Protein overexpression, extraction and purification by affinity and ion-exchange chromatography using HPLC (High Pressure Liquid Chromatography) ▪ Immunoblotting (Western Blot) ▪ Co-immunoprecipitation of protein complexes ▪ 2D Blue Native-PAGE – SDS-PAGE ▪ 2D IsoElectric Focusing (IEF) – SDS-PAGE ▪ Enzymatic activity assays ▪ MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization – Time Of Flight) mass spectrometry ▪ Antibiotic resistance E-test ▪ Swarming assays ▪ Methylation assays ▪ Mouse killing by perfusion, dissection, tissue homogenization ▪ Cryosectioning ▪ Microscopy ▪ Confocal Laser Microscopy ▪ Mammalian cell culture: generation of retroviruses, transduction, colony formation assays, long-term growth curves, microscopy, FACS analysis (Fluorescence-Activated Cell Sorting), BrdU assay |
Conferences and seminars
06.2018 | European Human Genetics Conference in conjunction with the European Meeting on Psychosocial Aspects of Genetics, 16-19 June, Milano, Italy |
03.2018 | Workshop on “Next Generation Diagnostics”, 22-24 March, Skopje, Macedonia |
01.2018 | COMBACTE Good Clinical Practice (GCP) Course, 1-31 January, CLIN-Net (online course) |
11.2017 | VI Conference for Rare Diseases in South-eastern Europe, 11 November 2017, Skopje, Macedonia (e-poster presentation) |
10.2017 | NUTRICON Congress 2017, 5-7 October, 2017, Skopje, Macedonia |
09.2017 | CoMBoS – 1st Congress of Molecular Biologists of Serbia, 20-22 September 2017, Belgrade, Serbia |
06.2014 | The Biology and Physics of Bacterial Chromosomes, 16-18 June 2014, Birmingham, UK (poster presentation) |
09.2013 | qPCR and digital PCR congress, 09-10 September 2013 Lyon, France |
03.2013 | ESF-EMBO Symposium: Bacterial Networks (bacnet13), 16-21 March 2013, Pultusk, Poland (poster presentation) |
03.2013 | MoLife Retreat, 25-26 March 2013, Bad Bevensen, Germany (oral presentation) |
05.2011 | MoLife Retreat (Molecular Life Sciences Retreat), 19-21 May 2011, Seefeld, Germany |
Publications
2018 | Stajkovska, A., Mehandziska, S., Stavrevska, M., Jakovleva, K., Nikchevska, N., Mitrev, Z., Kungulovski, I., Zafiroski, Gj, Tasic, V., and Kungulovski, G. (2018) Trio Clinical Exome Sequencing in a Patient With Multicentric Carpotarsal Osteolysis Syndrome: First Case Report in the Balkans. Front. Genet. 9:113. |
2017 | Mehandziska, S., Petrescu, A.M., and Muskhelishvili, G. (2017) Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins. In: The Bacterial Nucleoid: Methods and Protocols. (ed. Olivier Espeli) MiMB Springer Series |
2011 | Geertz, M., Travers, A., Mehandziska, S., Sobetzko, P., Janga, S. C., Shimamoto, N. and Muskhelishvili, G. (2011) Structural Coupling between RNA Polymerase Composition and DNA Supercoiling in Coordinating Transcription: a Global Role for the Omega Subunit? MBio, vol. 2 no. 4 e00034-11 |