10.2022 – to date

Head of the Laboratory of Genetics and Personalized Medicine,
Zan Mitrev Clinic, Skopje, North Macedonia

02.2017 – to date

Molecular Biologist in the Laboratory of Genetics and Personalized Medicine,
Zan Mitrev Clinic, Skopje, North Macedonia

09.2016 – 02.2017

Intern in the Laboratory of Genetics and Personalized Medicine
Special Hospital for Surgical Diseases Filip Vtori, Skopje

04.2016 – 09.2016

Intern in the Biochemical Laboratory
Special Hospital for Surgical Diseases Filip Vtori, Skopje

11.2012 – 04.2015

Resident Associate
College Nordmetall, Jacobs University Bremen, Bremen, Germany

03.2011 – 01.2015

Teaching Assistant in the Advanced Genetics Lab Course
Jacobs University Bremen, Bremen, Germany

06.2008 – 08.2008

Intern in the Molecular Genetics department, in the laboratory of Dr. Daniel Peeper
The Netherlands Cancer Institute (NKI-AVL), Amsterdam, Netherlands

07.2007 – 05.2009

Student laboratory assistant
Jacobs University Bremen, Bremen, Germany

07.2023

2022 VIP-A: Variant Interpretation Only Program, College of American Pathologists (online education)

07.2022

2021 VIP-A: Variant Interpretation Only Program, College of American Pathologists (online education)

11.2022

Symposium “HPV infections and associated diseases” from Macedonian HPV society and Macedonian society for sexually transferred diseases, 25-27 November 2022, Struga, Macedonia

07.2022

FEMS conference on Microbiology in association with Serbian Society for Microbiology, 30 June – 2 July, 2022, Belgrade, Serbia (oral poster presentation at a chaired poster session)

06.2019

COMBACTE GCP training, 15–18 June 2019, Podgorica, Montenegro

06.2019

European Human Genetics Conference, 15–18 June 2019, Gothenburg, Sweden (e-poster presentation)

06.2018

European Human Genetics Conference in conjunction with the European Meeting on Psychosocial Aspects of Genetics, 16-19 June 2018, Milano, Italy

03.2018

Workshop on “Next Generation Diagnostics”, 22-24 March 2018, Skopje, Macedonia

01.2018

COMBACTE Good Clinical Practice (GCP) Course, 1-31 January 2018, CLIN-Net (online course)

11.2017

VI Conference for Rare Diseases in South-eastern Europe, 11 November 2017, Skopje, Macedonia (e-poster presentation)

10.2017

NUTRICON Congress 2017, 5-7 October, 2017, Skopje, Macedonia

09.2017

CoMBoS – 1st Congress of Molecular Biologists of Serbia, 20-22 September 2017, Belgrade, Serbia

06.2014

The Biology and Physics of Bacterial Chromosomes, 16-18 June 2014, Birmingham, UK (poster presentation)

09.2013

qPCR and digital PCR congress, 09-10 September 2013 Lyon, France

03.2013

ESF-EMBO Symposium: Bacterial Networks (bacnet13), 16-21 March 2013, Pultusk, Poland (poster presentation)

03.2013

MoLife Retreat, 25-26 March 2013, Bad Bevensen, Germany (oral presentation)

05.2011

MoLife Retreat (Molecular Life Sciences Retreat), 19-21 May 2011, Seefeld, Germany

2022

Gjorgjievska,M., Mehandziska, S., Stajkovska, A., Pecioska-Dokuzovska, S., Dimovska, A., Durmish, I., Ismail, S., Pavlovska, T., Stojchevska, A., Ahmedi, H., Andonova, J., Nikolovska, M., Velickovikj, S., Mitrev, Z., Kungulovski, I., and Kungulovski G. (2021). Case Report: Omicron BA.2 Subvariant of SARS-CoV-2 Outcompetes BA.1 in Two Co-infection Cases. Front Genet. 10.3389/fgene.2022.892682

2020

Ugurov, P., Popevski, D., Gramosli, T., Neziri, D., Vuckova, D., Gjorgon, M., Stoicovski, E., Marinkovic, S., Veljanovska, L., Ignevska, K., Mehandziska, S., Ambarkova Vilarova, E., Mitrev, Z., and Rosalia, R. (2020).
Early Initiation of Extracorporeal Blood Purification Using the AN69ST (oXiris®) Hemofilter as a Treatment Modality for COVID-19 Patients: a Single-Centre Case Series. Brazilian Journal of Cardiovascular Surgery. 10.21470/1678-9741-2020-0403.

2020

Mehandziska, S., Stajkovska, A., Stavrevska, M., Jakovleva, K., Janevska, M., Rosalia, R., Kungulovski, I., Mitrev, Z., and Kungulovski, G. (2020)
Workflow for the implementation of precision genomics in healthcare. Front. Genet. 11. 10.3389/fgene.2020.00619.

2020

Markovska, M., Rosalia, R., Stajkovska, A., Mehandziska, S., Janevska, M., Kungulovski, I., Mitrev, Z., Kungulovski, G (2020).
Genetic aberrations in chromatin factors are pervasive, mutually exclusive, associated with a high mutational burden and improved response to checkpoint immunotherapy in cancer. 10.21203/rs.2.20947/v1.

2019

Stajkovska, A., Mehandziska, S., Rosalia, R., Stavrevska, M., Janevska., M., Markovska, M., Kungulovski, I., and Kungulovski, G. (2019)
A Pediatric Case of Glioblastoma Multiforme Associated With a Novel Germline p.His112CysfsTer9 Mutation in the MLH1 Gene Accompanied by a p.Arg283Cys Mutation in the TP53 Gene: A Case Report. Front. Genet. 10. 952. 10.3389/fgene.2019.00952.

2018

Klinceva, M., Mehandziska, S., Idoski, E., Stajkovska, A., Stavrevska, M., Jakovleva, K., Ampova-Sokolov, V., Rosalia, R., Kungulovski, G., Blazevska, M., Hristov, N., Milev, I., and Mitrev, Z. (2018) P961CYP2C19-dependent clopidogrel resistance is a possible cause for coronary stent and peripheral bypass occlusion. European Heart Journal. 39. 10.1093/eurheartj/ehy564.P961.

2018

Stajkovska, A., Mehandziska, S., Stavrevska, M., Jakovleva, K., Nikchevska, N., Mitrev, Z., Kungulovski, I., Zafiroski, Gj, Tasic, V., and Kungulovski, G. (2018) Trio Clinical Exome Sequencing in a Patient With Multicentric Carpotarsal Osteolysis Syndrome: First Case Report in the Balkans. Front. Genet. 9:113.

2017

Mehandziska, S., Petrescu, A.M., and Muskhelishvili, G. (2017) Isolation and Analysis of RNA Polymerase Supramolecular Complex with Associated Proteins. In: The Bacterial Nucleoid: Methods and Protocols. (ed. Olivier Espeli) MiMB Springer Series

2011

Geertz, M., Travers, A., Mehandziska, S., Sobetzko, P., Janga, S. C., Shimamoto, N. and Muskhelishvili, G. (2011) Structural Coupling between RNA Polymerase Composition and DNA Supercoiling in Coordinating Transcription: a Global Role for the Omega Subunit? MBio, vol. 2 no. 4 e00034-11

Diagnostics

Flow cytometry with Luminex® 100/200™ – expert level
Isolation of nucleic acids from blood, tissue and other biological samples, ctDNA isolation from liquid biopsy – expert level
Genotyping and pharmacogenetics analyses with real time polymerase chain reaction (PCR) – expert level
Genotyping pharmacogenetics analyses with StripAssays – expert level
Typization and quantification of infective microorganisms by real time PCR – expert level
Bioinformatic analyses and interpretation of whole exome, clinical exome and gene panels – expert level
NGS lab workflow with Ion Chef™ and Ion S5™ System – intermediate level
NGS lab workflow with Oxford Nano pore MinION – expert level
Genetic counselling – expert level
Biochemical analyses – proficient

Research

Molecular cloning
Bacterial phage transduction
PCR and qRT-PCR
In vitro transcription
Gel shift assays, EMSA (Electrophoretic Mobility Shift Assay)
Bacterial growth in flasks, in microplate reader, in fermentor
Protein overexpression, extraction and purification by affinity and ion-exchange chromatography using HPLC (High Pressure Liquid Chromatography)
Immunoblotting (Western Blot)
Co-immunoprecipitation of protein complexes
2D Blue Native-PAGE – SDS-PAGE
2D IsoElectric Focusing (IEF) – SDS-PAGE
Enzymatic activity assays
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization – Time Of Flight) mass spectrometry
Antibiotic resistance E-test
Swarming assays
Methylation assays
Mouse killing by perfusion, dissection, tissue homogenization
Cryosectioning
Microscopy
Confocal Laser Microscopy
Mammalian cell culture: generation of retroviruses, transduction, colony formation assays, long-term growth curves, microscopy, FACS analysis (Fluorescence-Activated Cell Sorting), BrdU assay

English

Serbian